Acinetobacter baumannii is a gramnegative bacterial pathogen associated with multidrug resistance and hospital outbreaks of infection, particularly in the intensive care unit. See also the main article on the acinetobacter genusacinetobacter baumannii is the most relevant human pathogen within the acinetobacter genus. Acinetobacter species were isolated from various clinical samples received in department of microbiology. Acinetobacter spp aspectos microbiologicos, clinicos y. The 1st international workshop on acinetobacter was held on 6th september, 1986, in manchester, england, in association with the 14th international congress of microbiology. Acinetobacter baumannii is rapidly emerging as a multidrugresistant pathogen responsible for nosocomial infections including pneumonia, bacteremia, wound infections, urinary tract infections, and meningitis. Group 2 carbapenems have been considered the agents of choice for the treatment of multiple drugresistant a. Amitava dasgupta, in microbiology and molecular diagnosis in pathology, 2017. Pdf chapter 1 specimen collection, processing, culture. The biology of acinetobacter taxonomy, clinical importance. This volume serves researchers studying the gramnegative bacterium acinetobacter baumannii with chapters detailing microbiological techniques, biochemical techniques, clinical samples, and next generation omics techniques to characterize the organism at the molecular level. See also the main article on the acinetobacter genus acinetobacter baumannii is the most relevant human pathogen within the acinetobacter genus.
Acinetobacter molecular biology caister academic press. Protein aggregation is associated with acinetobacter. It can be an opportunistic pathogen in humans, affecting people with compromised immune systems, and is becoming increasingly important as a hospitalderived infection. Normally nonpathogenic, acinetobacter can cause serious infections in critically ill patients. Historical introduction, position of bacteria, relationships to algae, yeasts, molds, protozoa, morphology, physiology, the study of bacteria. Buy acinetobacter molecular biology by ulrike gerischer from waterstones today. This reference explains the importance of these organisms, both from the patients viewpoint and the economic perspective, and provides clinicians with the knowledge they need to co. Still, several of the genera dealt with belong to the same family. You may not know that you have an acinetobacter infection until you get sick with one of the following. Apr, 2015 acinetobacter molecular biology, caister academic press. One of the characteristics of the acinetobacter species is the ability to survive in adverse environmental conditions 20,21.
The genus acinetobacter is a group of gramnegative, non motile and. Due to the prevalence of infections and outbreaks caused by multidrug resistant a. Acinetobacter baumannii is undoubtedly one of the most successful pathogens responsible for hospitalacquired nosocomial infections in the modern healthcare system. After identification, acinetobacter isolates were speciated and antibiotic susceptibility was determined by the standard disc diffusion method.
Acinetobacter sinusitis has been associated with the development of pneumonia, as the infected sinuses serve as reservoirs for dissemination to the lower respiratory tract. Genomics and molecular biology, caister academic press. The evolutionary trajectory of acinetobacter pathobiology b. Genome organization, mutation, and gene expression in acinetobacter. Methods and protocols aims to ensure successful results in the further study of this high priority area of antibiotic study. Rapid detection of acinetobacter baumannii and molecular. Global metabolic analyses of acinetobacter baumannii. Chapters detail microbiological techniques, biochemical techniques, clinical samples, and next generation omics techniques to characterize the organism at the molecular level.
Importance of acinetobacter spp overview of the microbial characteristics, taxonomy, and epidemiology of acinetobacter. Seasonal changes faced by the microbial communities inhabiting freshwater resources provide an opportunity to appraise the extent to which variation in the aquatic physicochemical variables affect the density and survival of the acinetobacter species. In addition, acinetobacter is uniquely suited to exploitation for biotechnological purposes. Classification of species within this genus has historically presented difficulty owing to the genetic diversity of this organism.
The ability to survive in adverse environmental conditions as well as high level of natural and acquired antimicrobial resistance make a. Mybook is a cheap paperback edition of the original book and will be sold at uniform, low price. Importance of acinetobacter spp overview of the microbial characteristics, taxonomy, and epidemiology of acinetobacter genome organization, mutation, and gene expression in acinetobacter molecular epidemiology of acinetobacter species typing acinetobacter strains. Renowned international authors have contributed chapters on diverse topics including taxonomy, genome diversity, oligonucleotide usage, polysaccharides, pathogenesis, virulence, biofilms. It emerged that there was wide agreement that the time was ripe to take stock of the entire biology of acinetobacter. Siderophores are low molecular weight compounds 4001,000 kda with high affinity for iron. Acinetobacter strains are often ubiquitous, exhibit metabolic versatility, are robust and some provide convenient systems for modern molecular genetic manipulation and subsequent product engineering. Review of acinetobacter baumannii as a highly successful.
To overcome this problem, knowledge of the pathogenesis. Acinetobacter molecular biology by ulrike gerischer waterstones. Keywords gramnegative bacterium human pathogens antibiotic resistance panresistant strains virulence a. The genus acinetobacter is a major cause of nosocomial infections. To trace the spread of acinetobacter baumannii in intensive care units icus before and after the 2008. Horizontal gene flow is a driving force in bacterial adap. Identification and characterization of type ii toxin. Acinetobacter baumannii is an important opportunistic pathogen associated with a variety of nosocomial infections. Authoritative and cuttingedge, acinetobacter baumannii. In gerischer u ed, acinetobacter molecular biology. The ten extended articles here follow suit in their descriptions of research in footandmouth disease viruses, the molecular biology of pestiviruses, arteriviruses, coronavirus replication and interaction with the host, hendra and nipah virus, the molecular mechanisms of pathogenesis and host range in avian influenza, molecular dissection of. Acinetobacter is a widespread microbe, present throughout the environment.
Overview of the microbial characteristics, taxonomy, and epidemiology of acinetobacter harald seifert, lenie dijkshoorn. Desiccation tolerance has been implicated as an important characteristic that potentiates the spread of the bacterial pathogen acinetobacter baumannii on dry surfaces. The 2nd international workshop on acinetobacter took place from 6th to 7th. Taxonomy, clinical importance, molecular biology, physiology, industrial. A total of 112 samples were collected from items in the hospital. Biocote some of the characteristics are as follows. The organisms covered in this chapter belong to a group of taxonomically and phylogenetically diverse, gramnegative nonfermentative rods and coccobacilli. Acinetobacter baumannii methods and protocols indranil biswas.
The study was conducted in tertiary care hospital, india over a period of 2 years. Fermentation of enzymatic reactions biochemical test of acinetobacter baumannii related notes. The genus acinetobacter is a group of gramnegative, nonmotile and. International symposium on the biology of acinetobacter. In this chapter we explore many of these characteristics and their potential applications including biodegradation and bioremediation, novel lipid and peptide production, enzyme engineering, biosurfactant and biopolymer production and engineering of novel derivatives of these products. Studies of virulence factors, pathogenesis, antimicrobial resistance, treatment options of a. The study was conducted at mustansiriyah university, college of science, department of biology. Culture media, methods of using culture media, isolation of bacteria in pure culture, study of the morphology of bacteria, study of the physiology of. Targeted gene replacement in acinetobacter baumannii springer.
Acinetobacter molecular biology books in microbiology. At the macroscale level, we find that desiccation tolerance is influenced by cell density and growth phase. Acinetobacter, of the order pseudomonadales and belonging to the gammaproteobacteria, comprise a group of microorganisms from soil and. Publishing industry library and information science science and technology, general. Acinetobacter baumannii has emerged as a significant hospital pathogen, quickly becoming resistant to. The genus acinetobacter is a group of gramnegative, nonmotile and nonfermentative bacteria belonging to the family moraxellaceae. Acinetobacter baumannii is a leading cause of nosocomial infections with increased morbidity and mortality. Acinetobacter baumannii an overview sciencedirect topics. There is currently increasing interest concerning the biology and disease caused by acinetobacter species.
Horizontal gene flow is a driving force in bacterial adap tation and has been shown to. Acinetobacter is a uniquely successful organism that is now presenting unusual challenges in terms of its detection and treatment in human disease. Such interest, however, developed relatively slowly because of the necessity to clarify the confusing taxonomy of these organisms. Pathogenesis, antibiotic resistance mechanisms, and prospective treatment options article pdf available in frontiers in cellular and infection microbiology 7. Biochemical test of acinetobacter baumannii online.
About this book introduction the 1st international workshop on acinetobacter was held on 6th september, 1986, in manchester, england, in association with the 14th international congress of microbiology. Distinguishing colony opacity variants and measuring. Methods and protocols methods in molecular biology biswas, indranil, rather. Much work was needed to identify various species as. Potential application of acinetobacter in biotechnology in acinetobacter molecular biology. Molecular characterization of invasive carbapenemresistant. Molecular biology by cornelis et al at over 30 bookstores. Biochemical test of acinetobacter baumannii image source. Nosocomial bloodstream infections caused by acinetobacter species in united states hospitals. Acinetobacter can cause nosocomial sinusitis in patients admitted to the intensive care unit. Acinetobacter has been in the focus of many studies addressing fundamental biological questions as well as its virulence potential.
Molecular detection of human bacterial pathogens 1 ed. Click and collect from your local waterstones or get free uk delivery on orders over. Taxonomy, clinical importance, molecular biology, physiology, industrial relevance 57 20, paperback at the best online prices at ebay. Table of contents hide biochemical test of acinetobacter baumannii some of the characteristics are as follows. Leeds acinetobacter medium is a novel antibioticcontaining selective medium that combines its selectivity with differential characteristics and has been used for the improved isolation of acinetobacter species from both clinical and environmental sources jawad etal. Acinetobacter biology and pathogenesis ebook ellibs. Renowned international authors have contributed chapters on diverse topics including taxonomy, genome diversity, oligonucleotide usage. Acinetobacter details the clinical aspects of this bacterium responsible for many infections in hospitalized patients. Biochemical analysis of enzymes responsible for increased antibiotic resistance in acinetobacter baumannii faculty mentor. Acinetobacter baumannii is a gramnegative, glucosenonfermenting, oxidasenegative coccobacillus, most commonly associated with the hospital settings.
Acinetobacter baumannii methods and protocols indranil. Itcan be isolated from many sources including soil, water, sewage, and food. Acinetobacter baumannii is a typically short, almost round, rodshaped coccobacillus gramnegative bacterium. In molecular biology and genetics, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings through the cell membranes. Molecular characterization of acinetobacter baumannii isolated from. Genome organization, mutation, and gene expression in acinetobacter l. They are important soil organisms where they contribute to the mineralisation of, for example, aromatic compounds. Althahab adepartment of biology, college of science, university of babylon, babylon, iraq bdepartment of biology, college of science, university of babylon, babylon, iraq abstract the nosocomial infections are major medical problems. Part of the methods in molecular biology book series mimb, volume 1946 abstract many strains of acinetobacter baumannii, including the highly virulent strain ab5075, undergo a highfrequency switch that results in two cell types that are distinguished by their opaque or translucent colony opacities when viewed by oblique lighting. Here we explore several factors influencing desiccation survival of a.
This book covers the following topics related to bacteriology. Genomic resistance island agi1 carrying a complex class 1 integron. Molecular typing of acinetobacter baumannii isolated from chinese. Molecular biology 1st edition by ulrike gerischer editor isbn. That occasion was so well attended and productive that there were soon discussions about how, when and where the next meeting should be held. Taxonomy, clinical importance, molecular biology, physiology, industrial relevance f. Genotyping of acinetobacter baumannii in nosocomial outbreak and surveillance. The organisms covered in this chapter belong to a group of taxonomically and phylogenetically diverse, gramnegative, nonfermentative rods and coccobacilli. The 12 th symposium on the biology of acinetobacter. The extraordinary competence of acinetobacter baylyi strain adp1 for. This reference explains the importance of these organisms, both from the patients viewpoint and the economic perspective, and provides clinicians with the knowledge they need to control these bacteria.
Acinetobacter baumannii infection what you need to know. This detailed volume serves clinicians and basic science researchers studying the increasingly antibiotic resistant gramnegative bacterium acinetobacter baumannii. It causes nosocomial infections, mostly ventilatorassociated pneumonia and bacteraemia, in immunocompromised and injured patients present in hospital intensive care units. Isolation and identification of acinetobacter species with. Acinetobacter molecular biology by ulrike gerischer. Free bacteriology books download ebooks online textbooks. Ebersberger1,3,4 1 applied bioinformatics group, institute of cell biology and neuroscience, goethe university frankfurt, 60438 frankfurt main, germany 2 institute of medical microbiology and infection control, hospital of the goethe university.
The genus acinetobacter constitutes a heterogeneous group of bacteria widespread in nature and known for its broad metabolic potential. That occasion was so well attended and productive that there were soon discussions about how, when and where the next meeting. This barcode number lets you verify that youre getting exactly the right version or edition of a book. Acinetobacter lwoffii is a known skin colonizer but has been found to cause bacteremia in hospitalized patients. All species are strictly aerobic, catalase positive, and oxidase negative. Basic characteristics properties acinetobacter baumannii.
Molecular analysis of the acinetobacter baumannii biofilm. Acinetobacter cells are gramnegative short rods coccobacilli, measuring 1. Acinetobacter baumannii can cause serious and sometimes lifethreatening infections. Acinetobacter baumannii complex has been shown to cause ventilatorassociated pneumonia and bloodstream infections. A rapid and sensitive molecular detection in clinical isolates is quite needed for the appropriate therapy and outbreak control of a. Read acinetobacter biology and pathogenesis by available from rakuten kobo.
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